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Somehow this series had become not Jordan vs. Ewing but Jackson vs. Jeff Van Gundy, the Knicks’ doleful coach. THOMAS ROBERTS, CNN STUDENT NEWS ANCHOR: Hello and welcome to this March 26th edition of CNN Student News! Glad to have you along I’m Thomas Roberts. Tension on the high seas: London decries the capture of 15 British troops, by Iranian forces. And a release seems nowhere on the horizon.
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Gene specific DNA methylation determinationGene specific DNA methylation was determined with a OneStep qMethyl Lite kit (Zymo Research, Irvine, CA, USA) and methyl primer (SABioscience, Frederick, MD, USA), with minor modifications. Promoter methylation for COX 2, BDNF, NF CREB, 12 LOX, p450 epoxygenase, synaptophysin and drebrin like genes were studied in AD, BD and control brains. Briefly, 20 of global DNA was incubated in the presence (test reaction) or absence (reference reaction) of methyl sensitive restriction enzymes (5 each) (BStUI, HpyCH4IV and HpaII, NEB, Ipswich, MA, USA) at 37 for 2 followed by real time reverse transcription PCR (RT PCR) as described in the manufacturer’s instructions.
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